Biochemistry
Selection of compounds with affinity for receptors through the use of biological assays and in vitro
- Studies of receptor binding with radioligands in vitro or ex vivo
- Studies of down-regulation and up-regulation of receptors
- Studies of dissociation of complex transducers coupled to receptors (assessment of agonistic / antagonistic properties)
- Determination of the ability of drugs to cross the blood-brain barrier
- Studies of metabolic stability within CNS
- Autoradiography technique, binding of radioligands in brain slices, to map receptors in the brain (psychotropic drugs)
- Enzyme Kinetics
- Quantification of the principal clinical-chemical blood parameters
- Evaluation of the profile of affinity, efficacy, potency of compounds with affinity for specific receptors through the use of ex vivo assays.
- Preparation of isolated organs (heart, blood vessels, intestinal muscle, excretory system, reproductive organs of rodents)
Equipment used:
- System for receptor binding
- System for isolated organs
- Multi-label plate reader
- Reader biological-clinical parameters